Open AccessInhibition of nitrate reductase and ATPase activities in Zea mays roots by tungsten and N, N'-dicyclohexylcarbodiimide
Author(s) -
J. Buczek,
Marek Burzyński,
A. Suder-Moraw
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1981.069
Subject(s) - chemistry , nitrate reductase , atpase , tungsten , zea mays , enzyme , nuclear chemistry , stereochemistry , biochemistry , biology , agronomy , organic chemistry
The activity of soluble and membrane-bound ATPase obtained from corn roots was in vivo markedly inhibited by N,N' -dicyclohexylcanbodiimide (DCCD) and W042- ions. DCCD (2.5 X 10-5 M) added to the nutrient solution strongly decreased in vivo nitrate reductase (NR) activity after 12-h growth of plants while it had no effect in experiments in vitro on NR activity. Tungsten in a concentration of 10-4 M completely blocked NR activity after 24 h. In the above used concentrations neither DCCD nor W042- inhibited completely N03- absorption by corn roots. The results suggest that there must exist in corn roots another or an additional mechanism of N03- assimilation apart from of that proposed by Butz and Jackson (1977)