Open AccessReduction of nitrates in Cucumis sativus L. seedlings I. Influence of tungsten and vanadium on absorption and reduction of nitrates
Author(s) -
J. Buczek,
Ewa Kowalińska,
Krystyna Kuczera
Publication year - 2014
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1980.023
Subject(s) - chemistry , vanadate , nuclear chemistry , tungsten , inorganic chemistry , organic chemistry
Investigations were conducted on the influence of tungsten (Na2WO4) and meta-vanadate (NaVO3) on uptake and reduction of nitrates by cucumber seedlings. Tungsten and vanadium almost completely inhibited nitrate reductase activity (NR) after treating the plants for 24 hours with nitrates in the presence of 10-4 M WO4-2 and 10-3 M VO3-. Uptake of NO3- in the presence of WO4-2 in this same period of time was lowered by about 50 %, while in the presence of VO3- by seventy percent. The ratio of NO3- absorbed to that reducted in control plants was around 3, whereas in the presence of WO4-2 and VO3- 9 and 8 respectively. The effect of NR inhibiting activity by WO4-2 and VO3- was significant but somewhat weaker if both inhibitors were applied to the plants 24 hours after formation of the NR system, whereas NO3- uptake was subject to a slight lowering. The above data suggest that WO4-2 and VO3- after a 24 hour application of these inhibitors to plants, primary inhibit biosynthesis and activity of NR and to a lesser extent effect NO3- absorption. On the basis of results reached the Butz and Jackson (1977) hypothesis concerning the model of NO3- uptake and reduction by plant tissues is discussed