Open AccessElectrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
Author(s) -
Aleksander F. Sikorski
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1979.042
Subject(s) - flagellum , chlamydomonas reinhardtii , chlamydomonas , electrophoresis , membrane , polyacrylamide gel electrophoresis , membrane protein , polyacrylamide , chemistry , gel electrophoresis , biology , biochemistry , chromatography , microbiology and biotechnology , gene , enzyme , mutant
SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides