Open AccessEffect of hydroxyurea on mitotic activity 3H-thymidine and 3H-phenylalanine incorporation in the antheridial filament cells of Chara vulgaris
Author(s) -
A. Bilecka
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1979.016
Subject(s) - mitosis , microbiology and biotechnology , meristem , biology , thymidine , vicia faba , dna synthesis , biochemistry , chemistry , dna , botany , gene
Hydroxyurea inhibits mitotic activity in cells of the antheridial filaments of Chara vulgaris by blocking phase S and phase G2. Blocking of cells in phase G2 also occurs in the case of the root meristem cells of Helianthus annuus and Vicia faba var. minor. 3H-thymidine incorporation confirmed autoradiographically the blocking of cells of the antheridial filaments in Chara vulgaris at phase S and slowing down of the rate of DNA replication. Incubation with 3H-phenylalanine demonstrated that hydroxyurea inhibits protein synthesis