Open AccessPolyacrylamide gel electrophoresis of soybean seed proteins
Author(s) -
W. Lassocińsk,
J. S. Knypl
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1978.033
Subject(s) - polyacrylamide gel electrophoresis , glycine , gel electrophoresis , polyacrylamide , electrophoresis , gel electrophoresis of proteins , molecular mass , chemistry , cultivar , chromatography , two dimensional gel electrophoresis , biology , biochemistry , botany , amino acid , microbiology and biotechnology , gene , proteomics , enzyme
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were detected under dissociating conditions. Molecular weights of three major protein fractions in PAGE SDS were determined for around 18 500, 36 000 and 80 000 daltons