Open AccessInfluence of hydroxyurea on nucleic acids content and 3H-uridine incorporation in callus and tumorous tobacco tissues cultured in vitro
Author(s) -
A. Bielecka
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1975.035
Subject(s) - callus , uridine , nucleic acid , in vitro , tissue culture , nicotiana tabacum , microbiology and biotechnology , dna , incubation , rna , biology , biochemistry , chemistry , botany , gene
In callus and tumor tissues of Nicotiana tabacum cultured for 39 days in media supplemented with various concentrations of hydroxyurea (1.3 x 10-4 M - 1.3 x 10-3 M) a decrease of DNA content (ca. 24 per cent in callus tissue and ca. 23 per cent in tumour tissue) and a decrease of RNA content (over 10 per cent and ca. 9 per cent in callus and tumour tissue, respectively) was observed. The autoradiographic method showed that a long-lasting action of this com-pound inhibits RNA synthesis. A stronger inhibitory influence of hydroxyurea upon incorporation of 3H-uridine from the incubation medium was revealed