Open AccessEffectiveness of different methods of preparing seedlings of cereals under sterile conditions for investigations of nucleic acids using radioactive precursors
Author(s) -
W. Filek
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1975.032
Subject(s) - chloramphenicol , sterilization (economics) , bacteria , sodium hypochlorite , germination , streptomycin , microorganism , chemistry , nucleic acid , antibiotics , sowing , food science , horticulture , biology , microbiology and biotechnology , biochemistry , genetics , organic chemistry , monetary economics , economics , foreign exchange market , foreign exchange
On seedlings grown from grain not sterilized in advance bacteria numbered more than 108 microorganisms per one gram of fresh weight. Washing of seedlings with cetyltrimethylammonium bromide or sodium lauryl sulphate solutions reduced the number of bacteria several times. Sterilization of grains prior to planting with sodium hypochlorite and germination on solid substratum (perlit) reduced the number of bacteria to below 104 microorganisms to one gram of fresh weight. If germination was, however, in water, bacteria on the seedlings were approximately as numerous as on seedlings from mon-sterilized grains. Of the three antibiotics tested (streptomycin, chloramphenicol, penicillin) the most effective against the bacteria of wheat seedlings was chloramphenicol coupled with streptomycin; of antibiotics used singly chloramphenicol was best