Open AccessAttempts at establishing the culture conditions for Lemna minor L.
Author(s) -
M. Krzychowska,
J. Zimna,
Krystyna Bytniewska,
W. Macjejewska-Potapczyk
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1975.021
Subject(s) - lemna minor , frond , lemna , botany , substrate (aquarium) , dry matter , horticulture , biology , chemistry , aquatic plant , ecology , macrophyte
The influence of the concentration, composition and pH of the substrate as well as of light intensity on the growth and vegetative propagation of Lemna minor L. was investigated. The media of Hutner, Hoagland and Pirson and Seidel were used. At first the experiments were carried out under unsterile conditions. Later sterilized duckweed was cultured in aseptic conditions. The dry matter was determined. Surface area increment and an increase in the number of fronds were evaluated by the planimetric method. For total protein determination in Lemna minor L. from unsterile and sterile cultures Lowry's method was used