Open AccessThe ultrastructure of protein bodies isolated from Pisum sativum and Iris pseudoacorus L. seeds
Author(s) -
Barbara Gabara,
L. Konopska
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1974.027
Subject(s) - pisum , sativum , ultrastructure , biology , sucrose , isolation (microbiology) , iris (biosensor) , acid phosphatase , botany , biochemistry , enzyme , microbiology and biotechnology , computer security , computer science , biometrics
Protein bodies of Pisum sativum and Iris pseudoacorus seeds have been isolated in sucrose gradient with addition of 50mM citrate buffer, pH 5. Their ultrastructure due to isolation procedure has been described. Two types of protein bodies are present in pea and iris seeds: simple and compex ones - with many inclusions. The method of isolation, used in this paper extracts partly proteins - probably albumins, and also substances present in globoids i.e. phytin and acid phosphatase