Open AccessEffect of morphactine on differentiation and development of Cymbidium Sw. protocorms cultured in vitro
Author(s) -
K. Kukułczanka,
B. Twarda-Pędota
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1973.021
Subject(s) - cymbidium , meristem , shoot , biology , in vitro , botany , agar , thickening , tissue culture , horticulture , chemistry , biochemistry , genetics , polymer science , bacteria
The morphogenetic effect of morphactine IT 3456 on meristematic tissue of Cymbidium Sw. was investigated within the dose range 0.01 to 10 ppm in in vitro culture on liquid and agar-solidified medium. In the doses applied, morphactine increased the number of protocorms differentiating from isolated meristematic tissues, and, in dependence on the dose, affected their shape and proliferation. Morphactine retards, and in large doses completely inhibits, the development of rhizoids, shoots and roots. Various developmental anomalies were observed under the influence of morphactine: leaf syncotylia, and their deformation, thickening, shortening, flattening and branching of shoots as well as formation of 2-3 shoots by one protocorm and development of secondary protocorms on the leaves