Open AccessActivity and localization of some hydrolytic enzymes during the development of Iris pseudoacorus endosperm
Author(s) -
Barbara Gabara,
L. Konopska,
Maria Olszewska
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1972.017
Subject(s) - endosperm , enzyme , acid phosphatase , biochemistry , hydrolysis , biology , cytochemistry , iris (biosensor) , microbiology and biotechnology , chemistry , cell wall , botany , computer security , computer science , biometrics
The changes in the activity of some hydrolytic enzymes during the development of Iris pseudoacorus endosperm were investigated using biochemical and cytochemical methods. In the early stages of development the chalazal pole shows a greater enzymatic activity than the micropylar pole. These differences decline as the seeds mature, and the activity of the studied enzymes becomes lower as the endosperm develops. Considerable activity of ß-galactosidase has been observed at the time of deposition of storage hemi-celluloses in the cell walls of the endosperm. Activity of the cytochemically datectable -hydrolases is localised in granules up to 2μ in diameter. Cytochemical observations in the electron microscope indicate that the activity of acid phosphatase is associated with spherosomes