Open AccessAttempts at DNP isolation from callus and tumour tissues of Nicotiana tabacum
Author(s) -
L Kłyszejko-Stefanowicz,
A. Rennert,
W. Mazurczyk,
Marian Wysocki,
W. Potaczyk
Publication year - 2015
Publication title -
acta societatis botanicorum poloniae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 29
eISSN - 2083-9480
pISSN - 0001-6977
DOI - 10.5586/asbp.1971.024
Subject(s) - callus , nicotiana tabacum , dna , tissue culture , nicotiana , nap , biology , rna , botany , microbiology and biotechnology , chemistry , solanaceae , biochemistry , in vitro , gene , neuroscience
This paper deals with a preliminary investigation of ONP originating from tumour and callus tissues of Nicotiana tabacum. DNP preparations from tumour tissue contained on the average: 54.88% of protein, 38.4% of DNA and 2,9% of RNA. The N/P ratio value fluctuated within the limits 4.8—5.8. The respective mean values for DNP preparations from callus tissue were: 57.3; 21.6 and 13.l%. The NAP ratio was 4.7-6.6