Attempts at DNP isolation from callus and tumour tissues of Nicotiana tabacum | Zendy

L Kłyszejko-Stefanowicz | Zendy; A. Rennert | Zendy; W. Mazurczyk | Zendy; Marian Wysocki | Zendy; W. Potaczyk | Zendy

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Attempts at DNP isolation from callus and tumour tissues of Nicotiana tabacum

Author(s) -

L Kłyszejko-Stefanowicz,

A. Rennert,

W. Mazurczyk,

Marian Wysocki,

W. Potaczyk

Publication year - 2015

Publication title -

acta societatis botanicorum poloniae

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.297

H-Index - 29

eISSN - 2083-9480

pISSN - 0001-6977

DOI - 10.5586/asbp.1971.024

Subject(s) - callus , nicotiana tabacum , dna , tissue culture , nicotiana , nap , biology , rna , botany , microbiology and biotechnology , chemistry , solanaceae , biochemistry , in vitro , gene , neuroscience

This paper deals with a preliminary investigation of ONP originating from tumour and callus tissues of Nicotiana tabacum. DNP preparations from tumour tissue contained on the average: 54.88% of protein, 38.4% of DNA and 2,9% of RNA. The N/P ratio value fluctuated within the limits 4.8—5.8. The respective mean values for DNP preparations from callus tissue were: 57.3; 21.6 and 13.l%. The NAP ratio was 4.7-6.6

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