Open AccessSome characteristic of Rhizoctonia spp. in sharp eyespot of wheat
Author(s) -
Helena Furgał-Węgrzycka,
Jan Adamiak,
E. Adamiak
Publication year - 2014
Publication title -
acta mycologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.38
H-Index - 5
eISSN - 2353-074X
pISSN - 0001-625X
DOI - 10.5586/am.1996.020
Subject(s) - eyespot , rhizoctonia , biology , virulence , pathogenicity , hypha , horticulture , rhizoctonia solani , microbiology and biotechnology , botany , gene , genetics
Isolates of Rhizttctonia sp. with multinucleate and binucleate cells were obtained from sharp eyespot lesions on wheat culms in Olsztyn region. (NE Poland). These isolates were compared to isolates of AG-4 and GAG-1 testers with reference to cultural morphology of colony, growth rate, hyphal anastomosis and pathogenicity to wheat seedlings. The wheat binucleate isolates were similar in morphology of colonies and anastomosed with the Ceratubasidium anastomosis group GAG-1 tester isolates of R. cerealis. Growth rates on PDA ranged from 9 to 11 mm/24h for wheat isolates and from l to 11 mm/24 h for tester isolates GAG-1 of R. cerealis. The wheat multinucleate isolates were similar in morphology of colonies and anastomosed with Rhizoctonia solani Kühn group AG-4 tester isolate. R. solani AG-4 isolates were morphologically distinct from the R. cerealis isolates. These isolates on PDA were dark and grow rapidly (20-30 mm diam./24 h/20°C) and significantly contrasted with slowly growing white-creamy isolates of R. cerealis (GAG-1). Isolates of R. solani (AG-4) and R. cerealis (GAG-I) developed sharp eyespot lesions on culms and white head symptoms typical of the disease. None of the wheat isolates of R. cerealis (GAG-I) caused root-rot on wheat seedlings. In the present work the classification system of vegetative groups of Rhizoctonia spp. in present work is also discussed