Open AccessFungi transporting by sowing seed material of herbs
Author(s) -
Z. Machowicz-Stefaniak,
Beata Zimowska
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.2000.012
Subject(s) - penicillium , biology , alternaria , phoma , botrytis , fusarium , agar , rhizopus , botany , potato dextrose agar , horticulture , exigua , agar plate , food science , botrytis cinerea , bacteria , fermentation , biochemistry , genetics , spodoptera , gene , recombinant dna
Sowing seed material of33 species of herbs obtained in 1997-1999 from the Herb Seed-Testing Station, in Bydgoszcz were examined. Fungi were isolated using the method of artificial cultures on the mineral medium. Sixty seeds superficially disinfected and sixty undisinfected seeds were taken from each sample. Obtained single-spore cultures of the fungi grown on malt-agar or on standard medium were identified up to the species level. Fungi species belonging to the genus Fusarium were identified on the PDA and SNA, Aspergillus spp. and Penicillium spp. on the malt-agar and Czapek-Dox and Phoma spp. on the malt-agar, oat-meal-agar and cherry-agar. Mycological analyses showed that the superficial disinfection of seeds reduced by three times the number of isolates obtained. The fungi most frequently isolated from both the inside and the outside seed tissues were Botrytis cinerea, Phoma exigua var. exigua and species of Alternaria, Epicoccum, Fusarium, Penicillium, Phyllosticta, Rhizopus, Trichothecium