The stability of electron- and immunoelectron microscopic plant virus preparations negatively stained with uranyl acetate and practical aspects of using this stain | Zendy

Marek S. Szyndel | Zendy

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The stability of electron- and immunoelectron microscopic plant virus preparations negatively stained with uranyl acetate and practical aspects of using this stain

Author(s) -

Marek S. Szyndel

Publication year - 2013

Publication title -

acta agrobotanica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 9

eISSN - 2300-357X

pISSN - 0065-0951

DOI - 10.5586/aa.1992.004

Subject(s) - uranyl acetate , uranyl , immunoelectron microscopy , stain , aqueous solution , chemistry , resolution (logic) , nuclear chemistry , chromatography , materials science , electron microscope , analytical chemistry (journal) , staining , physics , biology , optics , computer science , pathology , organic chemistry , medicine , antibody , ion , artificial intelligence , immunology

The electron microscopic preparations of tobamo-, potex-, carla-, poty-, clostero-, como-, cucumo-, ilar-, luteo- and cryptoviruses were completed in 1983-87 and stored at room temp. Grids were prepared by immunoelectron microscopic (clumping, ISEM, decoration) techniques and by leaf-dip modified method. All preparations were contrasted with 2 % aqueous uranyl acetate (UA), pH 4,2. Reexaminated grids were still of good quality. UA gives high contrast, high resolution and good preservation of the virus particles and a clear image of serological reactions. The advantages and disadvantages of UA and some practical aspects of using this stain are discussed

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