Open AccessThe stability of electron- and immunoelectron microscopic plant virus preparations negatively stained with uranyl acetate and practical aspects of using this stain
Author(s) -
Marek S. Szyndel
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1992.004
Subject(s) - uranyl acetate , uranyl , immunoelectron microscopy , stain , chemistry , aqueous solution , resolution (logic) , high resolution , nuclear chemistry , electron microscope , analytical chemistry (journal) , chromatography , staining , physics , optics , pathology , organic chemistry , computer science , medicine , geology , immunohistochemistry , ion , artificial intelligence , remote sensing
The electron microscopic preparations of tobamo-, potex-, carla-, poty-, clostero-, como-, cucumo-, ilar-, luteo- and cryptoviruses were completed in 1983-87 and stored at room temp. Grids were prepared by immunoelectron microscopic (clumping, ISEM, decoration) techniques and by leaf-dip modified method. All preparations were contrasted with 2 % aqueous uranyl acetate (UA), pH 4,2. Reexaminated grids were still of good quality. UA gives high contrast, high resolution and good preservation of the virus particles and a clear image of serological reactions. The advantages and disadvantages of UA and some practical aspects of using this stain are discussed