Open AccessIn vitro micropropagation of chive (Allium schoenoprasum L.)
Author(s) -
M. Lubomski
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1990.007
Subject(s) - shoot , micropropagation , explant culture , murashige and skoog medium , botany , sucrose , liliaceae , biology , allium , horticulture , tissue culture , in vitro , chemistry , food science , biochemistry
The investigations on micropropagation of Allium schoenoprasum embraced shoot formation from cultured shoot tips, shoot multiplication, root formations, and cold storage of rooted plants. All explants were cultured on modified Murashige-Skoog medium. Surface disinfection of shoots derived from stock plants was not necessary to obtain no infection culture. The highest shoot multiplication was obtained on medium with 20 g l-1 sucrose and 1.0 mg 1-1 6-benzylo-arninopurine (BA). Root formation was observed in both indoleacetic acid (IAA) and indolebutyric acid (IBA). Rooted plants were successfully kept in cold storage (5°C ± 1°C darkness). Only 20% of plants died after 6 months of storage