Open AccessElectrophoretic patterns of reserve seed proteins with reference to the taxonomic comparison of bean cultivars
Author(s) -
J. Czapski,
Ryszard Kkosson
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1986.018
Subject(s) - phaseolus , cultivar , globulin , biology , botany , genetic similarity , horticulture , genetic diversity , population , demography , sociology , immunology
The seed albumins and seed globulins of five Polish cultivars of Phaseolus vulgaris and one cultivar of Phaseolus multiflorus were electrophoretically separated in polyacrylamide gel using the disc-technique of Orstein and Davis. To compare any two cultivars, the percentage of similarity coefficient (S) was calculated based on the Rp values of protein bands. It was found that the electrophoretic mobility of the bean globulins is in general lower than albumins. Cultivar 'Piękny Jaś', belonging to Phaseolus multiflorus species showed large qualitative and quantitative differences in the albumin and globulin patterns as compared to other cultivars belonging to Phaseolus vulgaris species