Open AccessInfluence of water stress and abscisic acid on free proline accumulation in barley leaves
Author(s) -
Hanna Chełkowska,
Danuta Zielińska
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1983.006
Subject(s) - proline , wilting , abscisic acid , turgor pressure , horticulture , osmotic shock , water stress , chemistry , botany , biology , biochemistry , amino acid , gene
Rapid accumulation of free proline was found in barley leaves under conditions of osmotic stress at the level of -8.3 bars, but not before 9 days of its duration. The threshold value of osmotic stress for the start of a rapid accumulation of proline was about -6 bars. The excised leaves of barley subjected to water stress by wilting accumulated free proline more rapidly. In the course of 24 hours free proline content increased about 30-fold, while the water content in the leaves decreased 3-fold only. We found also that excised barley leaves with full turgor treated with abscisic acid (10-4 M) accumulated free proline as early as after 3 hours and subsequently after 24 hours free proline content increased 10-fold