Open AccessA method for rapid testing of the photosynthesis-inhibiting activity of herbicides by leaf disk infiltration
Author(s) -
Krzysztof Bielecki,
Halina Doroszewicz,
E. Grzyś,
Zdzisława Szuwalska
Publication year - 2013
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1982.013
Subject(s) - photosynthesis , mcpa , atrazine , phytotoxicity , chemistry , sodium carbonate , horticulture , sodium , botany , chromatography , agronomy , pesticide , biology , biochemistry , organic chemistry
A method for rapid detection of photosynthesis inhibitors in low concentration (0.25-1.25 ppm) was developed. The experiments were performed on disks cut from young bean leaves. The disks were infiltrated with solutions of the tested compounds and placed at the bottom of a crystallizer containing an acidic sodium carbonate solution and then illuminated. The toxicity of the tested substance was measured as the number of disks corning to the surface. It was found that linuron, monolinuron, metoksuron, atrazine and prometrine inhibited floating of the disks, whereas 2,4,5-T, MCPA and chlorpropham gave no effect. This confirms the specificity of the test which is appropriate for determining the phytotoxicity of typical photosynthesis inhibitors