Open AccessHaemolytic micromethod for rapid estimation of toxic alfalfa saponin
Author(s) -
Marian Jurzysta
Publication year - 2015
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1979.001
Subject(s) - saponin , trichoderma viride , chemistry , chromatography , food science , biology , botany , medicine , alternative medicine , pathology
On a plate covered with a thin layer of blood suspension with gelatine 10 microliters of alfalfa juice or alfalfa meal extract is spotted. As a result of saponin diffusion and their reaction with blood a haemolytic ring appears, the diameter of which is proportional to the concentration of toxic saponins. Selected by this method low-saponin individual alfalfa plants proved non toxic for fishes and did not inhibit the growth of Trichoderma viride