Haemolytic micromethod for rapid estimation of toxic alfalfa saponin | Zendy

Marian Jurzysta | Zendy

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Haemolytic micromethod for rapid estimation of toxic alfalfa saponin

Author(s) -

Marian Jurzysta

Publication year - 2015

Publication title -

acta agrobotanica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 9

eISSN - 2300-357X

pISSN - 0065-0951

DOI - 10.5586/aa.1979.001

Subject(s) - saponin , trichoderma viride , chemistry , chromatography , food science , biology , botany , medicine , alternative medicine , pathology

On a plate covered with a thin layer of blood suspension with gelatine 10 microliters of alfalfa juice or alfalfa meal extract is spotted. As a result of saponin diffusion and their reaction with blood a haemolytic ring appears, the diameter of which is proportional to the concentration of toxic saponins. Selected by this method low-saponin individual alfalfa plants proved non toxic for fishes and did not inhibit the growth of Trichoderma viride

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