Open AccessTissue culture of horse-radish (Cochlearia armoracia L.) meristems: sterilization of buds and comparison of media
Author(s) -
Krystyna Górecka,
W. Srzednicka,
Leszek S. Jankiewicz
Publication year - 2015
Publication title -
acta agrobotanica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 9
eISSN - 2300-357X
pISSN - 0065-0951
DOI - 10.5586/aa.1978.018
Subject(s) - agar , sterilization (economics) , horticulture , biology , botany , chemistry , business , genetics , finance , exchange rate , bacteria , foreign exchange market
The attempt was made to cultivate horse-radish meristems taken from buds removed from roots. The lowest per cent of contamination was found after the buds had been soaiked in 80% ethanol for 6 minutes and then in 5%, 7.5% or 10% chloramine for 30, 30 or 15 minutes, respectively. Both agar media: Murashige-Skoog and Linsmaier-Skoog, were equally good, providing a moderate number of developing plants. The Linsmaier-Skoog medium was more satisfactory when solidified with agar; the results with liquid medium and filter-paper bridges were not as good