Open AccessOptical coherence tomography reveals heterogeneity of the brain tissue and vasculature in the ischemic region after photothrombotic stroke in mice
Author(s) -
Hubert Doleżyczek,
Piotr Kasprzycki,
Jakub Włodarczyk,
Maciej Wojtkowski,
Monika Malinowska
Publication year - 2022
Publication title -
acta neurobiologiae experimentalis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 55
eISSN - 1689-0035
pISSN - 0065-1400
DOI - 10.55782/ane-2022-010
Subject(s) - optical coherence tomography , vascularity , medicine , microglia , angiogenesis , ischemia , pathology , ophthalmology , cardiology , inflammation
We demonstrate in vivo imaging of the ischemic area in the mouse brain after photostroke using a custom prototype Gaussian‑beamoptical coherence tomography (OCT) setup in which the near infrared imaging beam and the green photoinducing light pass throughthe same objective lens. The goal of our research was analysis of vascularity of the ischemic area during 2‑week progress of strokeand correlating the hypo‑ and hyperreflective OCT scattering areas with the location of activated microglia and astroglia. Angiogenesis,which was assessed using angiomaps, showed that the area of vessels in the ischemic center increased until day 7. OCT imagingrevealed a heterogeneous scattering signal pattern in the ischemic area. On structural OCT images, we found presence of a core area ofischemia with a hyporeflective OCT signal and a halo of hyperreflective signal around the core. The core signal decreased in size by 70%by day 14. Immunocytochemistry revealed that the hyporeflective area in the ischemic core was associated with microglia/macrophageactivation, whereas the hyperreflective signal from the halo came from activated astrocytes.