Open AccessIdentification of microRNA/target gene in the dentate gyrus of 7-day-old mice following isoflurane exposure
Author(s) -
Bing-Nan Yang,
Chu-Tong Zhang,
Lefan Liu,
Xiaolin Wu,
Haibo Hu,
Yu Chen,
Muneeb Iqbal,
Yong Ma,
Jinsong Zhou,
Xinli Xiao,
Jianxin Liu
Publication year - 2022
Publication title -
acta neurobiologiae experimentalis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 55
eISSN - 1689-0035
pISSN - 0065-1400
DOI - 10.55782/ane-2022-009
Subject(s) - dentate gyrus , hippocampal formation , neuroscience , neurotoxicity , kegg , axon guidance , biology , hippocampus , microrna , gene , axon , gene expression , medicine , genetics , transcriptome , toxicity
Studies on rodents and nonhuman primates suggest that exposure to anesthetics, particularly in the young brain, is associated withneuronal apoptosis as well as hippocampal-dependent cognitive dysfunction. Disruption of the development of dentate gyrus mayplay an important role in anesthetics-induced neurotoxicity. However, the anesthetics triggered molecular events in the dentate gyrusof the developing brain are poorly understood. By integrating two independent data sets obtained from miRNA‑seq and mRNA-seqrespectively, this study aims to profile the network of miRNA and potential target genes, as well as relevant events occurring in the dentategyrus of isoflurane exposed 7-day-old mice. We found that a single four hours exposure to isoflurane yielded 1059 pairs of differentlyexpressed miRNAs/target genes in the dentate gyrus. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichmentanalysis further indicates that dysregulated miRNAs/target genes have far‑reaching effects on the cellular pathophysiological events,such as cell apoptosis, axon development, and synaptic transmission. Our results would greatly broaden our functional understandingof the role of miRNA/target gene in the context of anesthetics-induced neurotoxicity.