Open AccessSynthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
Author(s) -
RAMAZAN PAŞAHAN,
Mitat Akkoç,
ŞEYMA YAŞAR,
TUĞBA KUL KÖPRÜLÜ,
ŞABAN TEKİN,
SEDAT YAŞAR,
İSMAİL ÖZDEMİR
Publication year - 2022
Publication title -
turkish journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.239
H-Index - 46
eISSN - 1303-6130
pISSN - 1300-0527
DOI - 10.55730/1300-0527.3418
Subject(s) - chemistry , hela , transmetalation , diethyl ether , carbene , ether , stereochemistry , cisplatin , medicinal chemistry , in vitro , organic chemistry , biochemistry , catalysis , medicine , surgery , chemotherapy
The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a-e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a-e). The Ru(II)-NHC complexes (4a-h) were synthesized via transmetalation reaction from 3a-e. 4a-h complexes were converted to Ru(II)-NHC.HCl complexes (5ah) by HCl solution of diethyl ether and characterized by different spectroscopic techniques such as 1 H and 13 C NMR, LC/MS-Q-TOF, FT-IR, elemental analysis, and melting point detection. We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a-h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay. The IC 50 value of 5b, 5c and 5e complexes exhibited good cytotoxic activity than cisplatin on C6 (14.2 ± 0.5 mM; 16.2 ± 0.4 mM; 24.2 ± 0.7 mM, respectively) and HeLa (11.1 ± 0.5 mM; 13.7 ± 0.3 mM; 22.8 ± 0.8 mM, respectively) cell lines.