Open AccessCytoplasmically localized tRNA-derived fragments inhibit translationin Drosophila S2 cells
Author(s) -
SYED MUHAMMAD HAMID,
BÜNYAMİN AKGÜL
Publication year - 2022
Publication title -
turkish journal of biology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 38
eISSN - 1303-6092
pISSN - 1300-0152
DOI - 10.55730/1300-0152.2610
Subject(s) - biology , transfer rna , translation (biology) , polysome , cytoplasm , microbiology and biotechnology , drosophila melanogaster , biogenesis , schneider 2 cells , gene , protein biosynthesis , genetics , function (biology) , drosophilidae , rna , ribosome , messenger rna , rna interference
Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5'-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila .