Open AccessХАВДАР ЭСИЙН ҮЙЛ АЖИЛЛАГААНД JSAP (JNK/STRESS-ACTIVATED PROTEIN KINASE-ASSOCIATED PROTEIN) УУРГИЙН ОРОЛЦОО
Author(s) -
Purev Erdenebaatar,
Н Риота,
Ё Кацүжи
Publication year - 2016
Publication title -
šinžlèh uhaany akademijn mèdèè/proceedings of the mongolian academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 2312-2994
pISSN - 2310-4716
DOI - 10.5564/pmas.v56i3.694
Subject(s) - centrosome , scaffold protein , microbiology and biotechnology , biology , signal transducing adaptor protein , mitosis , cancer cell , kinase , microtubule , cancer , cell cycle , signal transduction , genetics
JSAP1 and JSAP2 are structurally close related family proteins and originally have been identified as scaffold proteins of the JNK and p38 MAPK modules. The specificity of MAPK cascades is regulated, at least in part, by scaffold proteins, such as JSAPs[1-7]. Some studies showed [7-12] that JSAPs function as adaptor proteins which link cargoes to kinesin-1, the cellular cargo transporter along with microtubules. Also JSAPs regulate certain crucial neuronal processes, particularly axon elongation, branching and neurite outgrowth[13-22]. Interestingly, in recent studies it was reported that an elevated expression of JSAP2 protein in various cancers, including breast, colorectal cancer and hepatocellular carcinoma, might be proposed as a biomarker for diagnosis of cancers at early stage[25-28]. However, roles of JSAP in cancer cells and their underling molecular mechanisms are not known well at present. Within our project which aims to clarify potential roles of JSAPs in cancers, in the present study an overexpression of JSAPs in HeLa cells using lentiviral vectors were performed and immunocytochemical analyses focused on centrosomes at mitotic stage of dividing cells were done. In results, in HeLa dividing cells, which overexpress the wild type of JSAPs or their substitution mutant forms a multiple centrosome at mitotic stage of cells were found. However, in case of overexpression of the deletion mutant JSAP proteins in cancer cells a multi-centrosome abnormality were not observed, as well as in control cells.