Open AccessЦАХИЛДАГНЫ ТӨРЛИЙН УРГАМЛЫН ФИЛОГЕНЕТИК ХОЛБОО
Author(s) -
Х Алтанзул,
Ю Оюунбилэг
Publication year - 2016
Publication title -
šinžlèh uhaany akademijn mèdèè/proceedings of the mongolian academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 2312-2994
pISSN - 2310-4716
DOI - 10.5564/pmas.v56i3.685
Subject(s) - biology , phylogenetic tree , botany , genetics , gene
The phylogenetic relationship of eight Iris species (I.flavisimma, I.ventricosa, I.bungei,I.kaempferi, I.tenuifolia, I.lactea, I.tigrida, I.dichotoma) were studied by PCR using six specificprimers (S-523, Z1204R, trnL, trnF and ITS1, ITS4). All species except I.ventricosa wereidentified when using S-523, Z1204R and trnL, trnF primers and the DNA fragment size rangedbetween 500 to 600 bp and 800 bp, respectively. Primers ITS1 and ITS4 produced double bandsin about 500 and 1500 bp to I.bungei, I.kaempferi, and I.lactea species. On the phylogenetic tree, some iris species have close relations based on chloroplastic DNA sequences. The closest relationships showed between I.sanguinea and I.sibirica, I.rutenica and I.uniflora. Also I.tenuifolia, I.ventricosa and I.halophila located on one branch of phylogenetic tree.