Open AccessHigh Resolution Structure of the Manganese Derivative of Insulin
Author(s) -
Biserka Prugovečki,
Ivana Pulić,
Martina Toth,
Dubravka Matković-Čalogović
Publication year - 2012
Publication title -
croatica chemica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 50
eISSN - 1334-417X
pISSN - 0011-1643
DOI - 10.5562/cca2108
Subject(s) - manganese , derivative (finance) , insulin , chemistry , resolution (logic) , medicine , computer science , business , artificial intelligence , organic chemistry , finance
The structure of the human manganese derivative of insulin was determined using the single crystal X-ray diffraction method. Single crystals were grown by the hanging drop vapour diffusion crystallization method using zinc-free insulin and manganese(II) sulphate monohydrate in citrate buffer at pH = 6.4. It crystallizes in the trigonal system in the space group R3 with the unit cell parameters: a = b = 81.70 Å and c = 33.73 Å. There are two manganese ions per insulin hexamer in the human manganese-insulin derivative. The manganese ions lie on the three-fold crystallographic axis on opposite sides of the hexamer. Both manganese ions are octahedrally coordinated by three Nε2 atoms from the imidazolyl side chains of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules. The investigated human manganese-insulin derivative adopts the T6 conformation. (doi: 10.5562/cca2108