Expression, Purification and Crystallization of Wheat Profilin (Tri a 12) | Zendy

Christina Ecker | Zendy; Peter Forstenlechner | Zendy; D. Milić | Zendy; Karin HoffmannSommergruber | Zendy; Margit CichnaMarkl | Zendy; Dubravka MatkovićČalogović | Zendy

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Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)

Author(s) -

Christina Ecker,

Peter Forstenlechner,

D. Milić,

Karin HoffmannSommergruber,

Margit CichnaMarkl,

Dubravka MatkovićČalogović

Publication year - 2011

Publication title -

croatica chemica acta

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.214

H-Index - 50

eISSN - 1334-417X

pISSN - 0011-1643

DOI - 10.5562/cca1790

Subject(s) - crystallization , profilin , chemistry , expression (computer science) , microbiology and biotechnology , biochemistry , biology , computer science , organic chemistry , actin cytoskeleton , cytoskeleton , cell , programming language

Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees

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