Open AccessExpression, Purification and Crystallization of Wheat Profilin (Tri a 12)
Author(s) -
Christina Ecker,
Peter Forstenlechner,
Dalibor Milić,
Karin HoffmannSommergruber,
Margit CichnaMarkl,
Dubravka Matković-Čalogović
Publication year - 2011
Publication title -
croatica chemica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 50
eISSN - 1334-417X
pISSN - 0011-1643
DOI - 10.5562/cca1790
Subject(s) - crystallization , profilin , chemistry , expression (computer science) , microbiology and biotechnology , biochemistry , biology , computer science , organic chemistry , actin cytoskeleton , cytoskeleton , cell , programming language
Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coil, purified and crystallized using the hanging-drop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 angstrom. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 58.9 angstrom, c = 82.5 angstrom, alpha= beta = 90 degrees and gamma = 120 degrees