Open AccessHydrophilic Extractives in Heartwood of European Larch (Larix decidua Mill.)
Author(s) -
Janja Zule,
Katarina Čufar,
Vesna Tišler
Publication year - 2017
Publication title -
drvna industrija
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.287
H-Index - 19
eISSN - 1847-1153
pISSN - 0012-6772
DOI - 10.5552/drind.2016.1618
Subject(s) - larch , mill , pulp and paper industry , decidua , botany , forestry , chemistry , engineering , biology , geography , pregnancy , fetus , genetics , placenta
The heartwood of two European larch trees was examined for the content of hydrophilic extractives. Ethanol was used as a solvent for extractions of adequately pretreated samples, while gas chromatography with flame ionisation detection (GC-FID), gas chromatography coupled to mass spectrometry (GC-MS) and high performance size exclusion chromatography (HPLC-SEC) were applied for analyses. Different phenolic compounds, such as flavonoids and lignans were identified and quantitatively evaluated. The most abundant flavonoids were taxifolin and dihydrokaempferol, while the main lignan was secoisolariciresinol. Contents of flavonoids and lignans, measured at different stem heights, varied between 10.6 and 15.7 mg/g. At lower and medium heights flavonoids prevailed, while at upper stem positions the concentrations of lignans significantly increased. In addition, some trimeric and tetrameric phenolic structures were also detected. There was very little variation in the composition of hydrophilics between the two examined trees. The identified flavonoids and lignans are classified as very strong antioxidants.U radu su prikazani rezultati istraživanja hidrofilnih ekstraktivnih tvari u srži dvaju stabala europskog ariša. Kao otapalo za ekstrakciju prethodno adekvatno obrađenih uzoraka upotrijebljen je etanol, a za kemijske analize primijenjene su plinska kromatografi ja s plameno-ionizacijskim detektorom (GC-FID), plinska kromatografi ja povezana s masenom spektrometrijom (GC-MS) i tekućinska kromatografija visoke djelotvornosti (HPLC-SEC). U uzorcima su identificirani i kvantitativno procijenjeni različiti fenolni spojevi kao što su flavonoidi i lignani. Taksifolin i dihidrokamferol najzastupljeniji su flavonoidi, a glavni je lignan sekoizolaricirezinol. Cjelokupne koncentracije flavonoida i lignana koje su određivane u srži na različitim visinama stabla variraju između 10,6 i 15,7 mg/g. Na niskim i srednjim visinama prevladavaju flavonoidi, a na visinama bližima vrhu stabla zabilježen je značajan porast koncentracije lignana. Također, u ekstraktima su identificirani trimerni i tetramerni fenolni spojevi. Varijacije u sastavu hidrofilnih ekstraktiva između oba stabla bile su vrlo malene. Identificirani flavonoidi i lignani poznati su kao vrlo jaki antioksidansi