Open AccessIsolation of Bacillus From the Gut of Bombus terrestris and Its Correlation in Queen Mating
Author(s) -
Kai Li,
Liuhao Wang,
Zhengyi Zhang,
Yanjun Guo,
Jun Guo,
Nasar Amjad,
Hira Mannan Shaikh,
Jie Wu,
Jilian Li
Publication year - 2021
Publication title -
journal of agricultural science
Language(s) - English
Resource type - Journals
eISSN - 1916-9760
pISSN - 1916-9752
DOI - 10.5539/jas.v13n9p155
Subject(s) - mating , biology , bacillus pumilus , bacillus (shape) , zoology , bacillus cereus , bombus terrestris , bacteria , mating type , microbiology and biotechnology , botany , genetics , gene , pollen , pollinator , pollination
The gut of bumblebees harbors bacteria that play a crucial role in physiology, nutrition, and health. The mating rate is important for the reproductive activity of a colony; however, few studies have investigated the relationship between mating and gut bacteria. In this study, bacterial functions were identified in the intestinal tract of bumblebees, and biochemical identification and screening were performed using genetic detection technology. By isolating and identifying bacteria, we obtained a single strain and fed it to queens. The results indicated that Bacillus cereus and Bacillus pumilus are present in the gut. The queen mating rates were 48.89% at the period of 4 days and higher than 28.89% mating rates of the control group (P < 0.05), and the latency time were 16.90 min (from entering the mating cage to mating success) and decreased than control (P < 0.05) which was 28.20 min. This finding confirmed that Bacillus was important in Bombus terrestris mating.