Open AccessEffect of Deltamethrin on the Testicular Tissue of Albino Rats
Publication year - 2022
Publication title -
indian journal of ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.236
H-Index - 7
ISSN - 0304-5250
DOI - 10.55362/ije/2022/3522
Subject(s) - lumen (anatomy) , h&e stain , andrology , staining , atrophy , necrosis , testicular atrophy , biology , deltamethrin , pathology , endocrinology , medicine , pesticide , agronomy
This experiment aimed to investigate effect of changes of deltamerthrin on the testicular tissues of adult males' rat. Twenty-four adult male rats after their acclimatization for three weeks divided randomly and equally into three groups (n=8 rats): The LD50 of deltamerthrin was 150 mg kg . The experimental rats were inoculated orally with deltamerthrin 1/10 and 1/20 LD50 daily for 60 days. At the end of experiment -1animals were sacrificed post euthenization. For post mortem examination the testes were preserved in 10% formalin then processed with graduated concentrations of ethyl alcohol for impregnation in liquid paraffin then sectioned about 4-6 m stained with hematoxylin and eosin μstaining for microscopic examination. Deltamethrin with have significant Histopathological effects on male'stestes of rats. Hyper chromatic germ cells with rounded cells inside the lumen of seminiferous tubules with apoptotic cells, decreased mature sperms inside the lumen (hypospermatogensis), severe necrosis, damage of spermatogonial layers, atrophy and shrinkage of seminiferous tubules, reflected marked reduction of spermatozoa and decreased Leydig cells.