Open AccessANTIOXIDANT CAPABILITIES OF Litsea garciae BARK EXTRACTS AND THEIR RELATION TO THE PHYTOCHEMICAL COMPOSITIONS
Author(s) -
Siti Zaleha Raduan,
Qamar Uddin Ahmed,
Abdul Razak Kasmuri,
Muhamad Rusdi Ahmad Rusmili,
Md. Abdur Rashid Mia,
WAN MOHD AZIZI WAN SULAIMAN,
MUHAMMAD HAMDI MAHMOOD,
Mohd Farooq Shaikh
Publication year - 2022
Publication title -
malaysian applied biology/malaysian applied biology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 8
eISSN - 2462-151X
pISSN - 0126-8643
DOI - 10.55230/mabjournal.v51i1.2038
Subject(s) - phytochemical , chemistry , dpph , flavonoid , antioxidant , bark (sound) , traditional medicine , food science , composition (language) , chromatography , organic chemistry , biochemistry , biology , medicine , ecology , linguistics , philosophy
The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract's high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cis-feruloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.