
BIOACTIVITY OF BLACK CUMIN OIL ON THE SENESCENCE OF HER-2-OVEREXPRESSING BREAST CANCER CELLS
Author(s) -
Faradiba Nur Ahlina,
Lisyaratih Anggriani,
Irfani Aura Salsabila,
Riris Istighfari Jenie
Publication year - 2022
Publication title -
malaysian applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 8
eISSN - 2462-151X
pISSN - 0126-8643
DOI - 10.55230/mabjournal.v51i1.2008
Subject(s) - senescence , cytotoxicity , doxorubicin , cancer cell , breast cancer , chemistry , cancer research , chemotherapy , pharmacology , cell , cancer , in vitro , biology , microbiology and biotechnology , medicine , biochemistry
Senescence-induced therapy has been improved to increase its cytotoxicity and reduce the resistance of breast cancer cells to chemotherapy agents. An example of a potential senescence-inducing agent is black cumin oil (BCO) because one of its major compounds, α-pinene, can induce senescent cells. This study aims to explore the senescence-inducing activity of BCO in HER2-overexpressing breast cancer cells (MCF7/HER2). The yield obtained from hydro-distillation of BCO was 0.54%, and the main compounds were p-cymene (48.03%), dihydrocarveol (11.39%), and α-pinene (11.29%). BCO exhibited a moderate cytotoxicity profile indicated by IC50, which was >200 μg/mL in both cell lines. In combination with doxorubicin, BCO did not increase the cytotoxicity of doxorubicin. Moreover, BCO induced senescence by increasing 3% of the senescent cells compared with that of the control cells. However, this result was lower than that of the positive control on MCF7/HER2. BCO and doxorubicin combination increased the senescent cells by 3%–7% compared with the positive control on MCF7/HER2 cells. Therefore, the moderate cytotoxicity of BCO could be beneficial to the application of BCO as a supportive agent combined with a chemotherapy drug to increase cancer cells senescent and consequently inhibit cell proliferation.
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