Open AccessHYDROGEN PEROXIDE PROMOTES HUMAN SKIN FIBROBLAST CELLS MIGRATION AND PROLIFERATION AT LOWER DOSE VIA VEGF- AND TGF- GENE EXPRESSION
Author(s) -
Nur Aziz,
Johnson Stanslas,
Nurul Huda Abd Kadir
Publication year - 2020
Publication title -
malaysian applied biology/malaysian applied biology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 8
eISSN - 2462-151X
pISSN - 0126-8643
DOI - 10.55230/mabjournal.v49i4.1630
Subject(s) - fibroblast , wound healing , viability assay , reactive oxygen species , chemistry , cell migration , hydrogen peroxide , oxidative stress , gene expression , microbiology and biotechnology , dermal fibroblast , cell growth , transforming growth factor , cell , gene , biology , immunology , biochemistry , in vitro
Hydrogen peroxide (H2O2) and oxygen (O2), reactive oxygen species (ROS) that are formed during wound healing. Excessive exposure to H2O2 may lead to oxidative stress in cells. However, the low or moderate dosage of H2O2 might help in the wound healing process. AlamarBlueTM cell viability, scratch assay, and qRT-PCR analysis were done to evaluate cell viability, cell migration and gene expression of VEGF- and TGF- on the skin fibroblast cells treated with H2O2 (0 to 50 µM), respectively. Our results have shown that the fibroblast cells were significantly proliferated and migrated after treated with H2O2 (12.5 µM, 25 µM, and 50 µM) at 48h and 72h of exposure when compared to control. Furthermore, VEGF- gene expression on treated fibroblast cells (50 µM) was found to be higher than control, whereas TGF- gene expression was slightly lower than control. This result suggesting that cell proliferation and migration was possibly due to the activation of VEGF- but not TGF- gene.