Open AccessCLONING AND in silico STUDIES OF LIPASE GENE OF A THERMOPHILIC BACTERIA ISOLATED FROM THE HYDROTHERMAL VENT AREA OF KAWIO, NORTH SULAWESI
Author(s) -
KHALILAN LAMBANGSARI,
Maelita Ramdani Moeis,
Elvi Restiawaty
Publication year - 2021
Publication title -
malaysian applied biology/malaysian applied biology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 8
eISSN - 2462-151X
pISSN - 0126-8643
DOI - 10.55230/mabjournal.v49i2.1517
Subject(s) - lipase , thermophile , biology , 16s ribosomal rna , hydrothermal vent , bacteria , homology (biology) , gene , phylogenetic tree , microbiology and biotechnology , biochemistry , genetics , hydrothermal circulation , enzyme , paleontology
Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated from marine water was a member of family I.4 of ‘true lipase’ group.