SYNTHESIS, CHARACTERIZATION AND IN-VITRO RELEASE STUDY OF MUTUAL PRODRUGS OF MESALAMINE AND SULPHONAMIDES AS AZO COMPOUNDS

Azo compounds studied as prodrugs of sulfacetamide and sulfathiazole were prepared by diazotization reaction in which sulfacetamide and sulfathiazole were coupled with salicylic acid. The newly synthesized azo compounds were inoculated with Pseudomonas aeruginosa bacterium species which secrete the azoreductase enzyme causing the release of the parent compound. These results have been utilized to propose that azoreductase enzyme acts for the reduction of -N=N-. The mechanism of the reaction is discussed on the basis of release in terms of the formation of compounds like sulfacetamide/sulfathiazole and mesalamine. The use of bacteria as an enzyme producing agent, and released compounds acting as the anti-ulcer agent can find a variety of applications for colon targeting agents in the field of medicinal chemistry.