Open AccessSTUDY OF COLLAGENASE PRODUCTION BY PENICILLUM SP. ISOLATED FROM DETERIORATED LEATHER SAMPLE
Author(s) -
Savita Kate,
Archana Pethe
Publication year - 2022
Publication title -
journal of advanced scientific research
Language(s) - English
Resource type - Journals
ISSN - 0976-9595
DOI - 10.55218/jasr.202213126
Subject(s) - collagenase , gelatinase , enzyme , chemistry , substrate (aquarium) , incubation , biochemistry , food science , pulp and paper industry , biology , ecology , engineering
Collagenase production by Penicillium sp. isolated from deteriorated leather sample was studied. The isolate produced collagenase 802.43±15.81U/ml at optimum temperature 30ºC and at pH 6.5 within 5 days using 1% collagen peptide type I as a substrate. Ca++ ions were found to be the best activator whereas EDTA and β-mercaptoethanol inhibited collagenase production. The isolate was also found to produce caseinase, gelatinase and keratinase. Experimental results propose that the isolate having ability to produce broad range of enzymes can be effectively used as a tool for leather industry waste treatment.