The metal effect on the Polyphenol oxidase enzyme from borage(Trachystemon orientalis) plant | Zendy

Esma Hande Alıcı | Zendy; Gülnur Arabacı | Zendy

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The metal effect on the Polyphenol oxidase enzyme from borage(Trachystemon orientalis) plant

Author(s) -

Esma Hande Alıcı,

Gülnur Arabacı

Publication year - 2013

Publication title -

sakarya university journal of science

Language(s) - English

Resource type - Journals

eISSN - 2147-835X

pISSN - 1301-4048

DOI - 10.5505/saufbe.2013.28247

Subject(s) - polyphenol oxidase , chemistry , polyphenol , botany , enzyme , biology , biochemistry , antioxidant , peroxidase

In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5°C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+, Mg2+, Zn2+, Cu2+, Ca2+, K+, but inhibited by Hg2+, Mn2+, Ni2+, Sn2+, Na+. Ba2+, Al3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations

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