Open AccessDetermination of 50% endpoint titer using a simple formula
Author(s) -
Muthannan Andavar Ramakrishnan
Publication year - 2016
Publication title -
world journal of virology
Language(s) - English
Resource type - Journals
ISSN - 2220-3249
DOI - 10.5501/wjv.v5.i2.85
Subject(s) - simple (philosophy) , serial dilution , titer , mathematics , medicine , statistics , calculus (dental) , immunology , pathology , philosophy , alternative medicine , dentistry , epistemology , antibody
Two commonly used methods for calculating 50% endpoint using serial dilutions are Spearman-Karber method and Reed and Muench method. To understand/apply the above formulas, moderate statistical/mathematical skills are necessary. In this paper, a simple formula/method for calculating 50% endpoints has been proposed. The formula yields essentially similar results as those of the Spearman-Karber method. The formula has been rigorously evaluated with several samples.