Open AccessAntifungal Effect of Brachyglottis repanda Ethanol Extract
Author(s) -
Chan Nam Yook,
Young Soon Na,
Hee Joung Choi,
Il Soo You,
Jong Min Baek,
Seung Hwa Baek
Publication year - 2010
Publication title -
toxicological research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.872
H-Index - 30
eISSN - 2234-2753
pISSN - 1976-8257
DOI - 10.5487/tr.2010.26.2.117
Subject(s) - cytotoxic t cell , ethanol , chemistry , antifungal , in vitro , fungus , ic50 , trichophyton , traditional medicine , pharmacology , biology , microbiology and biotechnology , biochemistry , medicine , botany
The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at 150 μg/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney) . However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 (IC50, > 62,500 ng/ml) . Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% CH3CN/H2O, 100% CH3CN, and 50% CH3CN/H2O (SM 2 at 150 μg/disk) . The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, IC50 10,051 ng/ml at 75 μg/disk) . These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.