Open AccessCloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis
Author(s) -
Jianfeng Huang,
Jian Zhang,
Ting Lei,
Xiaodong Chen,
Yan Zhang,
Lulu Zhou,
Yu An,
Zhilong Chen,
Ronghua Zhou,
Yang Zhou
Publication year - 2010
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2010.43.7.491
Subject(s) - chemerin , lipogenesis , adipokine , adipose tissue , adipocyte , endocrinology , medicine , chemistry , biology , insulin resistance , insulin
Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPAR/ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPAR/, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.