Open AccessSynergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21
Author(s) -
Yuan Deng,
Yongqing Li,
Xiongwei Fan,
Wuzhou Yuan,
Huaping Xie,
Xiaoyang Mo,
Yan Yan,
Junmei Zhou,
Yuequn Wang,
Xianli Ye,
Yongqi Wan,
Xiushan Wu
Publication year - 2010
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2010.43.6.432
Subject(s) - psychological repression , transfection , immunoprecipitation , transcriptional regulation , transcription factor , gene , microbiology and biotechnology , transcription (linguistics) , crystallin , cytoplasm , complementary dna , cdna library , biology , chemistry , gene expression , genetics , linguistics , philosophy
LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes alphaB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with alphaB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, alphaB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or alphaB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both alphaB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and alphaB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.