Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21 | Zendy

Yun Deng | Zendy; Yongqing Li | Zendy; Xiongwei Fan | Zendy; Wuzhou Yuan | Zendy; Huaping Xie | Zendy; Xiaoyang Mo | Zendy; Yan Yan | Zendy; Junmei Zhou | Zendy; Yuequn Wang | Zendy; Xianli Ye | Zendy; Yongqi Wan | Zendy; Xiushan Wu | Zendy

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Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

Author(s) -

Yun Deng,

Yongqing Li,

Xiongwei Fan,

Wuzhou Yuan,

Huaping Xie,

Xiaoyang Mo,

Yan Yan,

Junmei Zhou,

Yuequn Wang,

Xianli Ye,

Yongqi Wan,

Xiushan Wu

Publication year - 2010

Publication title -

bmb reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.511

H-Index - 77

eISSN - 1976-670X

pISSN - 1976-6696

DOI - 10.5483/bmbrep.2010.43.6.432

Subject(s) - psychological repression , transfection , immunoprecipitation , transcriptional regulation , transcription factor , gene , microbiology and biotechnology , transcription (linguistics) , crystallin , cytoplasm , complementary dna , cdna library , biology , chemistry , gene expression , genetics , linguistics , philosophy

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes alphaB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with alphaB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, alphaB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or alphaB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both alphaB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and alphaB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.

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