Open AccessTranscriptional activation of pref-1 by E2F1 in 3T3 L1 cells
Author(s) -
Yan Shen,
Yoon Mo Kim,
CheolHeui Yun,
Yeonsil Moon,
Sang Hoon Kim
Publication year - 2009
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2009.42.10.691
Subject(s) - e2f1 , gene knockdown , transcription factor , luciferase , adipogenesis , transcription (linguistics) , e2f , microbiology and biotechnology , electrophoretic mobility shift assay , reporter gene , transmembrane protein , chemistry , biology , gene expression , gene , transfection , biochemistry , philosophy , receptor , linguistics
The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.