Open AccessExpression and localization of the spermatogenesis-related gene, Znf230, in mouse testis and spermatozoa during postnatal development
Author(s) -
Hongxia Song,
Dan Su,
Ping Lü,
Jiang-Yan Yang,
Wei Zhang,
Yuan Yang,
Yunqiang Liu,
Sizhong Zhang
Publication year - 2008
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2008.41.9.664
Subject(s) - spermatogenesis , biology , acrosome , gene , sperm , motility , andrology , immunofluorescence , spermatocyte , gene expression , sperm motility , complementary dna , microbiology and biotechnology , immunohistochemistry , meiosis , genetics , antibody , endocrinology , immunology , medicine
Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.