Open AccessExpression of CD320 in human B cells in addition to follicular dendritic cells
Author(s) -
Wha-Jung Cho,
Jin-Suk Choi,
Chan Hum Park,
Sun-Ok Yoon,
Dooil Jeoung,
YoungMyeong Kim,
Jongseon Choe
Publication year - 2008
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2008.41.12.863
Subject(s) - germinal center , follicular dendritic cells , cd19 , cytoplasm , cd38 , microbiology and biotechnology , confocal microscopy , chemistry , flow cytometry , b cell , messenger rna , tonsil , nucleus , cell , biology , in vitro , antibody , gene , antigen presenting cell , immunology , biochemistry , cytotoxic t cell , stem cell , cd34
CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. Although CD320 is known to enhance proliferation of germinal center (GC) B cells, little other information is available. In this study, we investigated its cellular distribution in the GC. Confocal microscopy of human tonsil sections revealed co-localization of CD320 with CD19 and CD38 but not with CD3 indicating that GC B cells expressed CD320 in addition to FDC. In purified GC B cells, CD320 expression was inhibited in the nucleus, membrane and cytoplasm. Reverse transcriptase-polymerase chain reaction confirmed CD320 mRNA expression in B cells. These finding indicate that CD320 is expressed in B cells in addition to FDC, and that its GC activity may be more complicated than previously thought.