The Effect of Long-Term High Protein, Low Carbohydrate, and Low Fat Diet in CKMB Enzymes and Troponin T in Male Wistar Rats

Obesity is one of the diseases caused by unhealthy eating patterns and diets. An unhealthy diet can increase cholesterol levels in the blood which will then accumulate on the inner walls of blood vessels, causing atherosclerosis. This study aims to determine the effect of long-term high-protein, low-carbohydrate and low-fat diet on heart function by examining male CKMB & Troponin T levels of Rattus Norvegicus. This research was conducted experimentally in a laboratory with a Post-Test Control Design Group research design. The number of samples was 20 male wistar rats and divided into two groups (standard feed group) and the TPRKRL diet (high protein, low carbohydrate, low fat diet group). Blood was examined in the Hasanuddin University medical research center laboratory using the ELIZA method. The analysis used to determine the difference between the two groups was the Maan-Whitney test. The results showed that the macronutrient composition of the high-protein diet had significantly higher protein content and percentage and lower carbohydrates (protein 78.25% vs. 15.25%; CHO 10.5% vs. 52.2%) compared to the standard feed composition. Meanwhile, post-intervention blood and organ Troponin T levels in the treatment group were 25.45 ± 1.80 and 1022.52 ± 447.89 pg/ml, respectively. And post-intervention blood and organ Troponin T levels in the control group were 23.99 ± 1.24 and 1117.56 ± 324.44 pg/ml. The TPRLLK diet was able to significantly reduce body weight compared to the standard diet and the TPRLLK diet did not increase the enzyme markers of heart damage.