Open AccessBiological Screening and Chemical Profiling of Actinomycete Strains Isolated from Extreme Environment
Author(s) -
Imran Sajid,
Sana Bukhari,
Adeela Fatima,
Ashba Hassan
Publication year - 2017
Publication title -
lgu journal of life sciences
Language(s) - English
Resource type - Journals
eISSN - 2521-0130
pISSN - 2519-9404
DOI - 10.54692/lgujls.2017.0104141
Subject(s) - antimicrobial , biology , extreme environment , pseudomonas , microbiology and biotechnology , streptomyces , acinetobacter , agar diffusion test , bacteria , antibiotics , antibacterial activity , genetics
The aim of this study was to isolate the antibiotic producing actinomycetes strains from the desert soil of the District Bahawalpur, Punjab, Pakistan. A total of twenty one actinomycetes strains were isolated from desert soil and were characterized morphologically, physiologically and biochemically. The16S rRNA sequencing of three isolates (Q2-A, Q2-S and Q2-12) recognized them as different species of the genus Streptomyces. Conventional methods of biological screening including; cross streaked, agar plug and well diffusion assay were used to screen the bioactive actinomycetes strains against MDR bacterialpathogens. The remarkable antimicrobial activity against (multi-drug resistant) MDR pathogens was observed through the well diffusion method by using methanolic crude extracts of isolated actinomycetes. The techniques of TLC and HPLC were used for chemical screening. Overall the desert actinomycetesisolated in this study has the potential of inhibiting the multi-drug resistant pathogenic strains like MRSA, Acinetobacter and Pseudomonas etc. So the extreme desert environment holds the valuable actinomycetes in its habitat which could be valuable in getting new antimicrobial compounds.