Open AccessCONTRIBUTION TO MOLECULAR GENETIC CHARACTERIZATION OF HELLEBORUS MULTIFIDUS Vis. IN BOSNIA AND HERZEGOVINA
Author(s) -
Lejla Lasić,
Semir Dorić,
Jasna Hanjalić,
Jasmina Čakar,
Belma Kalamujić Stroil,
Gabrijela Radosavljević,
Naris Pojskić
Publication year - 2016
Publication title -
radovi šumarskog fakulteta univerziteta u sarajevu
Language(s) - English
Resource type - Journals
eISSN - 2490-3183
pISSN - 1512-5769
DOI - 10.54652/rsf.2016.v46.i2.71
Subject(s) - genetic diversity , biology , evolutionary biology , montenegro , dna barcoding , genetics , geography , medicine , population , environmental health , regional science
UDK: 582.675.1:575(497.6)
Helleborus multifidus Vis. is endemic Illyric-Adriatic species with distribution range in Italy, Slovenia, Croatia, Bosnia and Herzegovina, Montenegro and Albania. Although few studies reported different taxonomic categories for H. multifidus, this one is the first using molecular-genetic markers (trnL region and matK of chloroplast DNA and nuclear ITS1 and ITS2 region) for genetic characterization of H. multifidus presented at three localites in Bosnia and Herzegovina. The results revealed that PCR-RFLP on trnL intron was not informative for testing inter- or intrapopulation diversity. Contrary, analysis of matK, ITS1 and ITS2 sequences showed differences between populations from Trebinje region and Kupreško polje, pointing to the need to include additional analyses in order to confirm these findings.