Open AccessEffects of methanol extract of Lindera Myrhha on the melanin synthesis of B16f10 melanoma cells
Author(s) -
Le Quynh Loan,
Phạm Thị Thúy,
Nguyen Luong Hieu Hoa,
Lê Văn Minh,
Nguyễn Hữu Hùng,
Nguyễn Hữu Dũng
Publication year - 2019
Publication title -
tạp chí khoa học đại học sư phạm thành phố hồ chí minh
Language(s) - English
Resource type - Journals
ISSN - 2734-9918
DOI - 10.54607/hcmue.js.16.3.2459(2019
Subject(s) - tyrosinase , chemistry , melanin , methanol , ic50 , traditional medicine , melanoma , hyperpigmentation , in vitro , cytotoxicity , skin whitening , active ingredient , biochemistry , pharmacology , biology , organic chemistry , medicine , enzyme , genetics
To evaluate the potential of herbal speciesin treatment of hyperpigmentation, this study focuses on the effect of methanol extract of Lindera myrhha on the melanin synthesis in B16F10 melanoma cells. Lindera myrhha showed low cytotoxicity at even high concentration. The methanol extract of this plant could inhibit the melanin synthesis in the dose dependent manner. At the concentration of 200 μg/ml, it could inhibit 42.58% of melanin formation in the B16F10 melanoma cells. The methanol extract of Lindera myrhha slightly inhibited in vitro mushroom tyrosinase activity (5.45%) at a concentration of 200 μg/ml and had a significant inhibitory effect on cellular tyrosinase activivity (36.35%). This plant also showed high free radical scavenging activity with IC50 = 41 mg/ml. This is the first report on activity of this kind in Lindera myrhha. In conclusion, this plant could be used as an effective whitening ingredient in cosmetic products.